Journal article
Protein kinase A negatively regulates Ca 2 signalling in Toxoplasma gondii
AD Uboldi, ML Wilde, EA McRae, RJ Stewart, LF Dagley, L Yang, NJ Katris, SV Hapuarachchi, MJ Coffey, AM Lehane, CY Botte, RF Waller, AI Webb, MJ McConville, CJ Tonkin
Plos Biology | PUBLIC LIBRARY SCIENCE | Published : 2018
Abstract
The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca 2+ signalling activates motility, but how this is switched off after invasion is complete to allow for replication to begin is not understood. Here, we show that the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A catalytic subunit 1 (PKAc1) of Toxoplasma is responsible for suppression of Ca 2+ signalling upon host cell invasion. We demonstrate that PKAc1 is sequestered to the parasite periphery by dual acylation of PKA regulatory subunit 1 (PKAr1). Upon gen..
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Grants
Awarded by UK Research and Innovation
Funding Acknowledgements
National Health and Medical Research Council (grant number APP1123218, APP1047806, APP1025598). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Australian Research Council (grant number FT120100164, DE160101035). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Agence Nationale de la Recherche (grant number LABEX PARAFRAP ANR-11-LABX-0024). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. CNRS-INSERM-Fondation FINOVI. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Victorian State Government Operational Infrastructure Support and the Australian Government. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Medical Research Council (MRC) UK (grant number MR/M011690/1). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. China Council. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.